Biochemical Study of Some New Cephems and Selenacephems Based on 6H-1,3-Thiazines and 6H-1,3-selenazines

Several new and know 6-(4-substituted phenyl)-4-(4-substituted phenyl)-2-phenyl-6H-1,3-thiazine (or selenazine) (Z4B7, Z4D5, Z4B7' Z4D5') were prepared by the 1,4-Michael addition reaction of chalcone derivatives with thiobenzamide or phenylselenocarboxamide in basic medium (where chalcones was formed Claisen-Schimidt condensation aromatic aldehydes 4-substituted acetophenone presence sodium hydroxide). These 6H-1,3-thia- selenazine used to a series cephem selenacephem compounds (i.e. 7-chloro-4-(4-substituted phenyl)-2-(4-substituted phenyl)-6-phenyl-5-thia 5-selena)-1-azabicyclo[4.2.0]oct-2-en-8-one; AZ4B7, AZ4D5, AZ4B7' AZ4D5'). All compound characterized IR, 1H NMR, 13C mass spectroscopic techniques elemental analysis. The toxicity assayed via determination their LD50 value using Dixon's up down method. antibacterial activity tested vitro against Staphylococcus aureus, Bacillus, Escherichia coli Pseudomonas aeruginosa. Furthermore, antioxidant, anticancer DNA cleavage efficiency evaluated.